VISPR Visualization

Prerequisites
- MAGeCK output files (.vispr.yaml)
- RCAC HPC account (Negishi or Bell)
Objective

Visualize the MAGeCK-VISPR results generated on the cluster.

MAGeCK-VISPR is a comprehensive quality control, analysis, and visualization workflow for CRISPR/Cas9 screens. This guide provides a straightforward method for running the visualization component on an HPC cluster and viewing the results on your local machine.

1. Connect to HPC and start an interactive session¶

First, open a terminal on your local computer and connect to the HPC cluster. Then, request an interactive session on a compute node.

# Connect to a Purdue RCAC HPC cluster (e.g., Negishi)
ssh purdue.pete@negishi.rcac.purdue.edu
# where `purdue.pete` is your username
# Request an interactive node with 8 cores for 4 hours
# Replace "your_account" with your specific account/allocation name
salloc -N 1 -n 8 -t 4:00:00 -A your_account

Once the job is allocated, you'll be automatically connected to a compute node. Take note of the node's name (e.g., a446) as you will need it later.

You can also do echo $SLURM_JOB_NODELIST to see the node name.

2. Load the modules and start the VISPR server¶

Next, load the required software modules and navigate to the directory containing your MAGeCK-VISPR results.

# Load the necessary modules
ml biocontainers
ml mageck-vispr

# Navigate to your project directory
cd /path/to/your/mageck-output

# Find a port number that is not in use
python3 -c 'import socket; s=socket.socket(); s.bind(("", 0)); print(s.getsockname()[1]); s.close()'

This command will output a port number that is not currently in use. You can use this port number to run the VISPR server. In my case, it returned 45511, but yours may be different.

# Start the VISPR server using the Apptainer container
# Replace port number (45511) with the port number you found in the previous step
apptainer exec /apps/biocontainers/images/quay.io_biocontainers_mageck-vispr\:0.5.6--py_0.sif \
vispr server --port 45511 results/*.vispr.yaml

The server will start and indicate it's running on a specific port, 45511. Leave this terminal running.

Warning

The session should remain active and should not display any errors. Warning messages are normal, but if you see errors, please check the module versions or your input files.

Click to see example server output

Above command will output something like this:

Negishi HPC terminal output
$ apptainer exec /apps/biocontainers/images/quay.io_biocontainers_mageck-vispr\:0.5.6--py_0.sif \
vispr server --port 45511 results/*.yaml
Loading data.
  config = yaml.load(f)
Starting server.

Open:  go to 127.0.0.1:45511 in your browser.
Note: Safari and Internet Explorer are currently unsupported.
Close: hit Ctrl-C in this terminal.
 * Serving Flask app "vispr.server" (lazy loading)
 * Environment: production
   WARNING: This is a development server. Do not use it in a production deployment.
   Use a production WSGI server instead.
 * Debug mode: off

3. Forward the port to your local computer¶

To view the web interface, you need to create an SSH tunnel from the compute node to your local machine.

Open a new, second terminal on your local computer and run the following command.

Note

Use your local terminal

This SSH tunnel command must be run from your local machine, not the HPC node.

Windows: Use WSL or PowerShell with SSH support.
macOS/Linux: Use the default terminal.

# Replace <node_name> with the name of the node from step 1 (e.g., a446.negishi.rcac.purdue.edu)
# Replace <port> with the port number from the server output (e.g., 45511)
# Replace <your_username> and <hpc_login_node> with your details
ssh -L <local-port>:localhost:<remote-port> -J <username>@<hpc-hostname-> <your_username>@<node_name>

Example:

If your job is on node a446, the server is on port 45511, your username is pete, and you log into negishi.rcac.purdue.edu, the command would be:

ssh -f -N -L 8788:localhost:45511 -J pete@negishi.rcac.purdue.edu pete@a446.negishi.rcac.purdue.edu

Note

You will be asked to enter your password for the HPC login node (or use the ssh-key if you have set it up and login without any prompt). Since you will likely be connecting to the compute node for the very first time you will also be asked to confirm the authenticity of the host. Type "yes" to continue.

PowerShellTerminal

Figure 1: SSH tunnel command output (local PowerShell terminal is being used here)

SSH tunnel command breakdown¶

The following table explains the components of the SSH command used to tunnel port 45511 through a jump host:

Option	Description
`-f`	Forks SSH to the background (ideal for port forwarding).
`-N`	Tells SSH not to run remote commands (just tunnel).
`-L 8788:localhost:45511`	Forwards local port to the remote compute node port.
`-J pete@negishi.rcac.purdue.edu`	Jump host (Negishi login node).
`pete@a446.negishi.rcac.purdue.edu`	Target compute node running the server.

4. View the VISPR interface¶

After successfully establishing the SSH tunnel, you can now access the VISPR web interface from your local machine.

Open a browser and go to:

1	`http://localhost:8788`

Note

Replace 8788 with the local port number you used for forwarding

You should now see the interactive VISPR dashboard rendered locally!

VISPR-interface

Figure 2: VISPR interface loaded in a web browser