RNA-seq analysis¶
A full-day, in-person, hands-on RNA-seq workshop: you will take real sequencing data from raw reads through quality control, quantification, and differential expression on RCAC clusters, with instructors in the room.
Workshop details
- Date: Tuesday, October 6, 2026
- Format: Full day, hands-on
- Location: In person, West Lafayette (room details provided upon registration)
- Time: 8:30 AM – 4:00 PM EDT
- Registration: Register on luma
- Instructor: Arun Seetharam
Required before the workshop
Complete the lesson's Summary and Setup page before the workshop day. SSH access to the cluster and data staging must be done before the session starts.
Workshop materials¶
The workshop follows the lesson site RNA-seq in practice: Hands-on workshop on RCAC systems (Carpentries format, CC-BY 4.0). The lesson site is the canonical material for this workshop; this page does not duplicate it.
Prerequisites¶
- An RCAC cluster account and the ability to log in (HPC Orientation for Biologists)
- Basic Unix shell skills (HPC Exchange, Week 1)
- Comfort with basic R (R Skills for Biological Data)
- A laptop you can bring
What you will be able to do afterward¶
- Fetch public sequencing data and run read-level QC on RCAC clusters
- Quantify expression and run a differential expression analysis
- Filter, annotate, and visualize results as publication-ready tables and figures
Related guides¶
Background reading in the Life Sciences section: